Following a history and physical examination, the initial evaluation of the male partner involves at least one properly performed semen analysis. Ideally, semen should be collected by masturbation, after 2 to 3 days of abstinence, into a clean container provided by the facility and examined within an hour after collection. A 2nd sample is usually recommended in the event of an abnormal initial semen analysis. A comprehensive analysis includes an evaluation of sperm count, overall motility and proportion of normal sperm (morphology) present in the sample. The objective is to get a sense of the semen quality and determine the need for additional testing including hormonal or genetic testing or referral to a specialist in male reproduction/urologist.