Intracytoplasmic Sperm Injection

Intracytoplasmic sperm injection (ICSI) is used to enhance the fertilization phase of in vitro fertilization (IVF) by injecting a single sperm into a mature egg. Rather than simply mixing the sperm and egg together, ICSI ensures that the sperm successfully penetrates the egg.

Following standard IVF ovarian stimulation and egg retrieval, sperm is collected from the male partner via masturbation or in some situations, acquired surgically from the male reproductive tract (testicle). Under high powered microscopy, a glass pipet is used to hold the egg in place and an injection pipet is used to inject one sperm into the egg.

ICSI is traditionally used to treat male infertility but is also used in unexplained infertility and previous failed IVF.